What Is Deep Well Plate

Feb 11, 2022

On the basis of the appearance size of ordinary microplates (mainly 96 and 384-well plates), while keeping the length and width in line with SBS international standards, the depth of the wells is increased to achieve the purpose of increasing the volume of each well. And in order to adapt to its specific range of use, on the one hand, by changing the manufacturing materials (polypropylene (PP) is currently used, and polystyrene (PS) is used individually), on the other hand, by improving the surface treatment process, a class of experiments are made. Room Plate.

1. According to the number of holes, the more common ones can be divided into 96-well plates and 384-well plates.

2. According to the hole type, the 96-well plate can be mainly divided into round hole type and square hole type. The 384-well plates are all square wells.

3. According to the shape of the bottom of the hole, there are two common types: U-shaped and V-shaped.

Scope of use

① To store samples:

It can replace the conventional 1.5ml centrifuge tube to store samples, and it can be neatly placed during storage, saving space, and has a large storage capacity, which can withstand -80 ℃ refrigerator. Therefore, it is also called a storage block.

② Processing samples:

It can be combined with a discharge gun, high-throughput automatic liquid manipulation instruments and software to achieve high-throughput manipulation of biological samples, such as protein precipitation and liquid-liquid extraction. Greatly improve the efficiency of sample processing. PP material can withstand 121 ℃ high temperature and high pressure sterilization.

③ Sample injection operation:

Commonly used in various autosamplers, it can be directly placed in the sample chamber of the autosampler for injection. Compared with the traditional sample injection vial, it can not only double the number of samples in the sample chamber, but also realize the sample storage in the sample chamber. The 96-well plate can be directly injected after processing, eliminating the tedious work of drawing samples back and forth, placing samples, covering lids, inserting cannulas, and washing bottles.