Usage And Precautions For Culture Dish

1. Precautions:

Before use, the culture dish is cleaned and disinfected. Whether it is clean or not has a significant impact on the work and can affect the acidity and alkalinity of the culture medium. If certain chemicals are present, they can inhibit bacterial growth.
Newly purchased culture dishes should be rinsed with hot water first, then soaked in a hydrochloric acid solution with a mass fraction of 1% or 2% for several hours to remove free alkaline substances, and then rinsed twice with distilled water.

To cultivate bacteria, use high-pressure steam (usually 6.8 * 10 to the 5th power Pa high-pressure steam) to sterilize at a temperature of 120 ° C for 30 minutes, dry at room temperature, or sterilize with dry heat. This means placing the cultured blood in an oven and maintaining the temperature at around 120 ° C for 2 hours to kill the bacterial cells.

Only sterilized petri dishes can be used for inoculation and cultivation.

2. Usage:

Place the reagent bottles that need to be used in a suitable position on the work area and loosen the caps of the reagent bottles that will be used soon;

Place the petri dish in the center of the work area;

Remove the cap of the reagent bottle and use a straw to remove the reagent from the bottle;

Place the lid of the culture dish behind the culture dish;

Gently inject the culture medium directly into the base of one side of the culture dish;

Cover the lid of the culture dish;

Place the culture dish on the side where it was originally placed, taking care not to let the medium enter the small gap between the lid and bottom;

Remove the used straw.