How To Properly Use A 96-well PCR Plate?

When using a 96 well plate for fluorescence quantitative PCR experiments, it is recommended to use an optical film instead to seal the reaction hole. The correct sealing method is to press the film along the longitudinal direction of the 96 hole plate, then laterally, and finally press along the edge of the plate to seal it· After adding the sample, some small liquid droplets will splash on the pipe wall, and there will also be some bubbles in the reaction system. It is necessary to remove the hanging liquid droplets and bubbles by centrifugation. Please use a dedicated microplate centrifuge to solve the above problems· The PCR board is a disposable consumable and is produced in one shot. It is not recommended to cut and use it, which can easily increase experimental errors and cannot be cleaned or partially reused. Don't bother with the follow-up analysis of the experiment in order to save a little money.